A rapid microassay to evaluate enzymatic hydrolysis of lignocellulosic substrates.
Identifieur interne : 003E80 ( Main/Exploration ); précédent : 003E79; suivant : 003E81A rapid microassay to evaluate enzymatic hydrolysis of lignocellulosic substrates.
Auteurs : Alex Berlin [Canada] ; Vera Maximenko ; Renata Bura ; Kyu-Young Kang ; Neil Gilkes ; Jack SaddlerSource :
- Biotechnology and bioengineering [ 0006-3592 ] ; 2006.
Descripteurs français
- KwdFr :
- Bois (MeSH), Catalyse (MeSH), Cellulases (composition chimique), Cellulases (métabolisme), Cellulose (composition chimique), Cellulose (métabolisme), Chromatographie en phase liquide à haute performance (MeSH), Glucose (analyse), Glucose (métabolisme), Hydrolyse (MeSH), Lignine (analyse), Lignine (composition chimique), Lignine (métabolisme), Liriodendron (composition chimique), Microchimie (méthodes), Oses (analyse), Papier (MeSH), Penicillium (enzymologie), Spécificité du substrat (MeSH), Trichoderma (enzymologie), Éthanol (composition chimique).
- MESH :
- analyse : Glucose, Lignine, Oses.
- composition chimique : Cellulases, Cellulose, Lignine, Liriodendron, Éthanol.
- enzymologie : Penicillium, Trichoderma.
- métabolisme : Cellulases, Cellulose, Glucose, Lignine.
- méthodes : Microchimie.
- Bois, Catalyse, Chromatographie en phase liquide à haute performance, Hydrolyse, Papier, Spécificité du substrat.
English descriptors
- KwdEn :
- Catalysis (MeSH), Cellulases (chemistry), Cellulases (metabolism), Cellulose (chemistry), Cellulose (metabolism), Chromatography, High Pressure Liquid (MeSH), Ethanol (chemistry), Glucose (analysis), Glucose (metabolism), Hydrolysis (MeSH), Lignin (analysis), Lignin (chemistry), Lignin (metabolism), Liriodendron (chemistry), Microchemistry (methods), Monosaccharides (analysis), Paper (MeSH), Penicillium (enzymology), Substrate Specificity (MeSH), Trichoderma (enzymology), Wood (MeSH).
- MESH :
- chemical , analysis : Glucose, Lignin, Monosaccharides.
- chemical , chemistry : Cellulases, Cellulose, Ethanol, Lignin.
- chemical , metabolism : Cellulases, Cellulose, Glucose, Lignin.
- chemistry : Liriodendron.
- enzymology : Penicillium, Trichoderma.
- methods : Microchemistry.
- Catalysis, Chromatography, High Pressure Liquid, Hydrolysis, Paper, Substrate Specificity, Wood.
Abstract
Current attempts to produce ethanol from lignocellulosic biomass are focused on the optimization of pretreatment to reduce substrate recalcitrance and the improvement of enzymes for hydrolysis of the cellulose and hemicellulose components to produce fermentable sugars. Research aimed at optimizing both aspects of the bioconversion process involves assessment of the effects of multiple variables on enzyme efficiency, resulting in large factorial experiments with intensive assay requirements. A rapid assay for lignocellulose hydrolysis has been developed to address this need. Pretreated lignocellulose is formed into handsheets, which are then used to prepare small disks that are easily dispensed into microtiter plates. The hydrolysis of cellulose to glucose is estimated using an enzyme-coupled spectrophotometric assay. Using disks prepared from ethanol organosolv pretreated yellow poplar, it is shown that the assay generates data comparable with those produced by hydrolysis of pretreated yellow poplar pulp in Erlenmeyer flasks, followed by HPLC analysis of glucose. The assay shows considerable time and cost benefits over the standard assay protocol and is applicable to a broad range of lignocellulosic substrates.
DOI: 10.1002/bit.20783
PubMed: 16345088
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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sort="Maximenko, Vera" uniqKey="Maximenko V" first="Vera" last="Maximenko">Vera Maximenko</name></author><author><name sortKey="Bura, Renata" sort="Bura, Renata" uniqKey="Bura R" first="Renata" last="Bura">Renata Bura</name></author><author><name sortKey="Kang, Kyu Young" sort="Kang, Kyu Young" uniqKey="Kang K" first="Kyu-Young" last="Kang">Kyu-Young Kang</name></author><author><name sortKey="Gilkes, Neil" sort="Gilkes, Neil" uniqKey="Gilkes N" first="Neil" last="Gilkes">Neil Gilkes</name></author><author><name sortKey="Saddler, Jack" sort="Saddler, Jack" uniqKey="Saddler J" first="Jack" last="Saddler">Jack Saddler</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16345088</idno><idno type="pmid">16345088</idno><idno type="doi">10.1002/bit.20783</idno><idno type="wicri:Area/Main/Corpus">003F11</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" 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de la Colombie-Britannique</orgName><placeName><settlement type="city">Vancouver</settlement><region type="state">Colombie-Britannique </region></placeName></affiliation></author><author><name sortKey="Maximenko, Vera" sort="Maximenko, Vera" uniqKey="Maximenko V" first="Vera" last="Maximenko">Vera Maximenko</name></author><author><name sortKey="Bura, Renata" sort="Bura, Renata" uniqKey="Bura R" first="Renata" last="Bura">Renata Bura</name></author><author><name sortKey="Kang, Kyu Young" sort="Kang, Kyu Young" uniqKey="Kang K" first="Kyu-Young" last="Kang">Kyu-Young Kang</name></author><author><name sortKey="Gilkes, Neil" sort="Gilkes, Neil" uniqKey="Gilkes N" first="Neil" last="Gilkes">Neil Gilkes</name></author><author><name sortKey="Saddler, Jack" sort="Saddler, Jack" uniqKey="Saddler J" first="Jack" last="Saddler">Jack Saddler</name></author></analytic><series><title level="j">Biotechnology and bioengineering</title><idno type="ISSN">0006-3592</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Catalysis (MeSH)</term><term>Cellulases (chemistry)</term><term>Cellulases (metabolism)</term><term>Cellulose (chemistry)</term><term>Cellulose (metabolism)</term><term>Chromatography, High Pressure Liquid (MeSH)</term><term>Ethanol (chemistry)</term><term>Glucose (analysis)</term><term>Glucose (metabolism)</term><term>Hydrolysis (MeSH)</term><term>Lignin (analysis)</term><term>Lignin (chemistry)</term><term>Lignin (metabolism)</term><term>Liriodendron (chemistry)</term><term>Microchemistry (methods)</term><term>Monosaccharides (analysis)</term><term>Paper (MeSH)</term><term>Penicillium (enzymology)</term><term>Substrate Specificity (MeSH)</term><term>Trichoderma (enzymology)</term><term>Wood (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Bois (MeSH)</term><term>Catalyse (MeSH)</term><term>Cellulases (composition chimique)</term><term>Cellulases (métabolisme)</term><term>Cellulose (composition chimique)</term><term>Cellulose (métabolisme)</term><term>Chromatographie en phase liquide à haute performance (MeSH)</term><term>Glucose (analyse)</term><term>Glucose (métabolisme)</term><term>Hydrolyse (MeSH)</term><term>Lignine (analyse)</term><term>Lignine (composition chimique)</term><term>Lignine (métabolisme)</term><term>Liriodendron (composition chimique)</term><term>Microchimie (méthodes)</term><term>Oses (analyse)</term><term>Papier (MeSH)</term><term>Penicillium (enzymologie)</term><term>Spécificité du substrat (MeSH)</term><term>Trichoderma (enzymologie)</term><term>Éthanol (composition chimique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Glucose</term><term>Lignin</term><term>Monosaccharides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cellulases</term><term>Cellulose</term><term>Ethanol</term><term>Lignin</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulases</term><term>Cellulose</term><term>Glucose</term><term>Lignin</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Glucose</term><term>Lignine</term><term>Oses</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Liriodendron</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cellulases</term><term>Cellulose</term><term>Lignine</term><term>Liriodendron</term><term>Éthanol</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Penicillium</term><term>Trichoderma</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Penicillium</term><term>Trichoderma</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Microchemistry</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellulases</term><term>Cellulose</term><term>Glucose</term><term>Lignine</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Microchimie</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Catalysis</term><term>Chromatography, High Pressure Liquid</term><term>Hydrolysis</term><term>Paper</term><term>Substrate Specificity</term><term>Wood</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Bois</term><term>Catalyse</term><term>Chromatographie en phase liquide à haute performance</term><term>Hydrolyse</term><term>Papier</term><term>Spécificité du substrat</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Current attempts to produce ethanol from lignocellulosic biomass are focused on the optimization of pretreatment to reduce substrate recalcitrance and the improvement of enzymes for hydrolysis of the cellulose and hemicellulose components to produce fermentable sugars. Research aimed at optimizing both aspects of the bioconversion process involves assessment of the effects of multiple variables on enzyme efficiency, resulting in large factorial experiments with intensive assay requirements. A rapid assay for lignocellulose hydrolysis has been developed to address this need. Pretreated lignocellulose is formed into handsheets, which are then used to prepare small disks that are easily dispensed into microtiter plates. The hydrolysis of cellulose to glucose is estimated using an enzyme-coupled spectrophotometric assay. Using disks prepared from ethanol organosolv pretreated yellow poplar, it is shown that the assay generates data comparable with those produced by hydrolysis of pretreated yellow poplar pulp in Erlenmeyer flasks, followed by HPLC analysis of glucose. The assay shows considerable time and cost benefits over the standard assay protocol and is applicable to a broad range of lignocellulosic substrates.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16345088</PMID><DateCompleted><Year>2006</Year><Month>05</Month><Day>24</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0006-3592</ISSN><JournalIssue CitedMedium="Print"><Volume>93</Volume><Issue>5</Issue><PubDate><Year>2006</Year><Month>Apr</Month><Day>05</Day></PubDate></JournalIssue><Title>Biotechnology and bioengineering</Title><ISOAbbreviation>Biotechnol Bioeng</ISOAbbreviation></Journal><ArticleTitle>A rapid microassay to evaluate enzymatic hydrolysis of lignocellulosic substrates.</ArticleTitle><Pagination><MedlinePgn>880-6</MedlinePgn></Pagination><Abstract><AbstractText>Current attempts to produce ethanol from lignocellulosic biomass are focused on the optimization of pretreatment to reduce substrate recalcitrance and the improvement of enzymes for hydrolysis of the cellulose and hemicellulose components to produce fermentable sugars. Research aimed at optimizing both aspects of the bioconversion process involves assessment of the effects of multiple variables on enzyme efficiency, resulting in large factorial experiments with intensive assay requirements. A rapid assay for lignocellulose hydrolysis has been developed to address this need. Pretreated lignocellulose is formed into handsheets, which are then used to prepare small disks that are easily dispensed into microtiter plates. The hydrolysis of cellulose to glucose is estimated using an enzyme-coupled spectrophotometric assay. Using disks prepared from ethanol organosolv pretreated yellow poplar, it is shown that the assay generates data comparable with those produced by hydrolysis of pretreated yellow poplar pulp in Erlenmeyer flasks, followed by HPLC analysis of glucose. The assay shows considerable time and cost benefits over the standard assay protocol and is applicable to a broad range of lignocellulosic substrates.</AbstractText><CopyrightInformation>(c) 2005 Wiley Periodicals, Inc.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Berlin</LastName><ForeName>Alex</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Department of Wood Science, Biotechnology and Biomaterials Group, Faculty of Forestry, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z4. alex.berlin@ubc.ca</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maximenko</LastName><ForeName>Vera</ForeName><Initials>V</Initials></Author><Author ValidYN="Y"><LastName>Bura</LastName><ForeName>Renata</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Kang</LastName><ForeName>Kyu-Young</ForeName><Initials>KY</Initials></Author><Author ValidYN="Y"><LastName>Gilkes</LastName><ForeName>Neil</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Saddler</LastName><ForeName>Jack</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biotechnol Bioeng</MedlineTA><NlmUniqueID>7502021</NlmUniqueID><ISSNLinking>0006-3592</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009005">Monosaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>11132-73-3</RegistryNumber><NameOfSubstance UI="C036909">lignocellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>3K9958V90M</RegistryNumber><NameOfSubstance UI="D000431">Ethanol</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.-</RegistryNumber><NameOfSubstance UI="D044602">Cellulases</NameOfSubstance></Chemical><Chemical><RegistryNumber>IY9XDZ35W2</RegistryNumber><NameOfSubstance UI="D005947">Glucose</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002384" MajorTopicYN="N">Catalysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D044602" MajorTopicYN="N">Cellulases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="N">Cellulose</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000431" MajorTopicYN="N">Ethanol</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005947" MajorTopicYN="N">Glucose</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031567" MajorTopicYN="N">Liriodendron</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008832" MajorTopicYN="N">Microchemistry</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009005" MajorTopicYN="N">Monosaccharides</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010209" MajorTopicYN="N">Paper</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010407" MajorTopicYN="N">Penicillium</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013379" MajorTopicYN="N">Substrate Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014242" MajorTopicYN="N">Trichoderma</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014934" MajorTopicYN="N">Wood</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>12</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>5</Month><Day>25</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>12</Month><Day>14</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16345088</ArticleId><ArticleId IdType="doi">10.1002/bit.20783</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Colombie-Britannique </li></region><settlement><li>Vancouver</li></settlement><orgName><li>Université de la Colombie-Britannique</li></orgName></list><tree><noCountry><name sortKey="Bura, Renata" sort="Bura, Renata" uniqKey="Bura R" first="Renata" last="Bura">Renata Bura</name><name sortKey="Gilkes, Neil" sort="Gilkes, Neil" uniqKey="Gilkes N" first="Neil" last="Gilkes">Neil Gilkes</name><name sortKey="Kang, Kyu Young" sort="Kang, Kyu Young" uniqKey="Kang K" first="Kyu-Young" last="Kang">Kyu-Young Kang</name><name sortKey="Maximenko, Vera" sort="Maximenko, Vera" uniqKey="Maximenko V" first="Vera" last="Maximenko">Vera Maximenko</name><name sortKey="Saddler, Jack" sort="Saddler, Jack" uniqKey="Saddler J" first="Jack" last="Saddler">Jack Saddler</name></noCountry><country name="Canada"><region name="Colombie-Britannique "><name sortKey="Berlin, Alex" sort="Berlin, Alex" uniqKey="Berlin A" first="Alex" last="Berlin">Alex Berlin</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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